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  • Detection of transovarial dengue virus from field-caught Aedes aegypti and Ae. albopictus larvae using C6/36 cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques. / Rohani, A;Zamree, I;Lee, H L.;Mustafakamal, I;Norjaiza, M J;Kamilan, D
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Detection of transovarial dengue virus from field-caught Aedes aegypti and Ae. albopictus larvae using C6/36 cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques. / Rohani, A;Zamree, I;Lee, H L.;Mustafakamal, I;Norjaiza, M J;Kamilan, D

Tác giả : Rohani, A;Zamree, I;Lee, H L.;Mustafakamal, I;Norjaiza, M J;Kamilan, D

Nhà xuất bản : WHO Regional Office for South-East Asia.

Năm xuất bản : 2007

Chủ đề : 1. Aedes Aegypti. 2. Aedes Albopictus. 3. Dengue. 4. Malaysia. 5. Journal / periodical articles.

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Tóm tắt :

Dengue Bulletin. 2007 Dec; 31: 47-57. Larvae of Aedes aegypti and Aedes albopictus were collected from a wide variety of artificial containers. Most samples were collected from used tyres and water-holding containers located in residential urban or rural areas. The identified mosquito larvae were pooled according to the species, date and locality and stored at –70 °C. A total of 378 pools of Ae. aegypti and 553 pools of Ae. albopictus were collected. Virus isolation was carried out using cell culture (C6/36 clone) of Ae. albopictus and virus detection by reverse-transcriptase polymerase chain reaction (RT-PCR). Transovarial transmission of dengue virus was demonstrated in both Ae. aegypti and Ae. albopictus in nature. Infected larvae were recovered from 16 localities (10 in Terengganu; 5 in Kuala Lumpur and 1 in Pahang). The study showed that both the cell culture and RT-PCR techniques can be used to detect dengue virus from mosquito larvae.
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https://iris.who.int/handle/10665/170453

 

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Transovarial Transmission of Dengue Virus in Aedes aegypti and Aedes albopictus in Relation to Dengue Outbreak in an Urban Area in Malaysia. / Lee, H L;Rohani, A

Tác giả : Lee, H L;Rohani, A

Nhà xuất bản : WHO Regional Office for South-East Asia

Năm xuất bản : 2005