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Molecular methods in plant disease diagnostics / Edited by Neil Boonham, Jenny Tomlinson and Rick Mumford

Tác giả : Edited by Neil Boonham, Jenny Tomlinson and Rick Mumford

Nhà xuất bản : CABI

Năm xuất bản : 2016

Nơi xuất bản : Boston, MA

Mô tả vật lý : xii, 200 pages : illustrations (black and white) ; 26 cm

ISBN : 9781780641478

Số phân loại : 632.3

Tùng thư : Principles and protocols

Chủ đề : 1. Bệnh cây -- Di truyền học. 2. Plant Diseases -- Genetics. 3. Chẩn đoán bệnh cây.

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Tóm tắt :

Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

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