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  • Statistical Optimization of Culture Media for Growth and Lipid Production of Botryococcus braunii LB572 / Choul-Gyun Lee
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Statistical Optimization of Culture Media for Growth and Lipid Production of Botryococcus braunii LB572 / Choul-Gyun Lee

Tác giả : Choul-Gyun Lee

Nhà xuất bản : Biotechnology and Bioprocess Engineering

Năm xuất bản : 2009-08-09

Chủ đề : 1. Botryococcus braunii LB572. 2. Central composite design. 3. Fatty acids. 4. Fractional factorial design. 5. Lipid. 6. Microalgae. 7. Article.

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Tóm tắt :

Botryococcus braunii has an outstanding ability to produce lipid; however, it is a slow-growing green microalgae. Statistical optimization of growth media was performed to faster growth and to increase lipid concentration. The effect of media composition on the growth of B. braunii LB572 was examined using fractional factorial design and central composite design. The media components examined include sodium carbonate, potassium phosphate, calcium chloride, magnesium sulfate, ferric citrate, and sodium nitrate. The results indicated that potassium phosphate and magnesium sulfate were major impact factors. The optimum concentrations of potassium phosphate and magnesium sulphate were found to be 0.058 and 0.09 g/L, respectively, for growth and 0.083 and 0.1 g/L, respectively, for lipid production. These values were validated using bubble column photobioreactors. Lipid productivity increased to 0.19 g/L/day in lipid-optimized media, with an average biomass productivity of 0.296 g/L/ day and 64.96% w/w. In growth-optimized media, lipid productivity was 0.18 g/L/day, with an average biomass productivity of 0.304 g/L/day and 59.56% w/w.
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Viện Vi sinh vật và Công nghệ Sinh học - ĐHQGHN
https://repository.vnu.edu.vn/handle/VNU_123/10587

 

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Sách, tuyển tập

Evaluation of Extraction Methods for Recovery of Fatty Acids from Botryococcus braunii LB 572 and Synechocystis sp. PCC 6803 / Choul-Gyun Lee

Tác giả : Choul-Gyun Lee

Nhà xuất bản : Biotechnology and Bioprocess Engineering

Năm xuất bản : 2009