Ethanol extracts of Scutellaria baicalensisprotect against lipopolysaccharide-induced acute liver injury in mice / Bui, Thanh Tung
Tác giả : Bui, Thanh Tung
Nhà xuất bản : Elsevier
Năm xuất bản : 2015
Chủ đề : 1. Cytokine. 2. Lipopolysaccharide. 3. Liver injury. 4. Nuclear factor-κB. 5. Scutellaria baicalensis. 6. Article.
Thông tin chi tiết
Tóm tắt : | Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods:Dried roots of S. baicalensiswere extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNAlevels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results:Liver injury induced by LPSsignificantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensisprevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions:Our study showed that S. baicalensisis potentially protective against LPS-induced liver injury in mice |
Thông tin dữ liệu nguồn
Thư viện | Ký hiệu xếp giá | Dữ liệu nguồn |
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Đại học Y - Dược - ĐHQGHN |
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https://repository.vnu.edu.vn/handle/VNU_123/11455 |