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  • Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions / Mustafa, A.S.;Abal, A.T.;Chugh, T.D.
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Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions / Mustafa, A.S.;Abal, A.T.;Chugh, T.D.

Tác giả : Mustafa, A.S.;Abal, A.T.;Chugh, T.D.

Năm xuất bản : 1999

Chủ đề : 1. Diagnosis, Differential. 2. DNA, Bacterial. 3. Mycobacterium. 4. Mycobacterium Infections. 5. Mycobacterium tuberculosis. 6. Polymerase Chain Reaction. 7. Reproducibility of Results. 8. Sensitivity and Specificity. 9. Tuberculosis.

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61-70 The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. The polymerase chain reactions differentiated between M. tuberculosis and non-tuberculous mycobacteria when standard strains and clinical isolates of mycobacteria were tested. The sensitivity of the two-band and three-band techniques to detect M. tuberculosis in clinical specimens, compared with smear and/or culture, was 88% and 75% respectively. Although both techniques showed 100% specificity, the superior sensitivity of the two-band technique suggests that it could be more useful in the diagnosis of tuberculosis and in differentiating M. tuberculosis complex from non-tuberculous mycobacteria
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https://iris.who.int/handle/10665/118685