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Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli / Jafari, A.;Katouli, M.;Shokouhi, F.;Bouzari, S.

Tác giả : Jafari, A.;Katouli, M.;Shokouhi, F.;Bouzari, S.

Năm xuất bản : 2001

Chủ đề : 1. Bacterial Adhesion. 2. Cell Culture Techniques. 3. Diarrhea. 4. DNA Fingerprinting. 5. DNA, Bacterial. 6. Drug Resistance, Multiple, Bacterial. 7. Escherichia coli Infections. 8. Escherichia coli O157. 9. F Factor. 10. Shiga Toxin 1. 11. Shiga Toxin 2. 12. Sorbitol.

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Tóm tắt :

756-762 The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates

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https://iris.who.int/handle/10665/119085